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HBO/WO

MBO

2020

Molecular Neurobiology, 3rd year BSc Psychobiology, UvA

Here you can find the basic course information necessary for the open inquiry course Molecular Neuroscience at the UvA. Molecular Neurobiology is part of the Genetics and Molecular Cell Biology learning trajectory. It is a 3rd year elective in which students get a crash course of 4 weeks in research design, execution, analysis, and interpretation. Students are divided in research-groups of 10-12 students under supervision of a ‘Principal Investigator’, who is assigned to a specific table and helps the student throughout the course with their ideas, execution, and interpretation. These PI’s are usually PhD students that will assist the students in designing their own research plan and experiments. Each research group receives a research topic (either ALS of Parkinson’s disease) and a toolbox to design and perform experiments with (LabBuddy and ExperD). Students have 4 weeks to execute their ideas, write a research report, and present their results and conclusions to their peers. The overall learning goal of this course is that students become familiar with the difficulties of scientific research. They need to become aware of what kind of information a specific model system can give you and what the specific strengths and shortcomings of different approaches and experiments are.

Website

Forrest Bradbury

2020

Resource list Molecular Neurobiology

The resources used for Molecular Biology are: Facilities: Practical rooms for labwork Practical rooms for cell culture Lecture halls Rooms for labmeetings Necessities: Protocols (see attached learning material (Reader Molecular Neurobiology)) Equipment and consumables (attached file) Software LabBuddy and ExperD as online reader and experiment designer (see link)

Spreadsheet

Simone Mesman

2020

Course Objectives Molecular Neurobiology

The overall learning goal of this course is that students become familiar with the difficulties of scientific research. They need to become aware of what kind of information a specific model system can give you and what the specific strengths and shortcomings of different approaches and experiments are. The attached document further specifies the learning goals of the course.

Document

Forrest Bradbury, Simone Mesman

2020

Course schedule & setup Molecular Neurobiology

In the Read-me file, you'll find a description of the set-up of the course and a crude planning of the course. The Excel file shows a detailed example schedule.

Document

Forrest Bradbury, Simone Mesman

August 30, 2016

DNA Electrophoresis – pouring a gel

Instruction on the preparation of an agarose gel for DNA electrophoresis Keywords: DNA, electrophoresis, separation, molecular biology, agarose, TAE

Video

Author unknown

August 30, 2016

DNA Electrophoresis – loading and running a gel

Instruction on how to load and run an agarose gel for DNA electrophoresis Keywords: DNA, electrophoresis, separation, molecular biology, agarose, TAE

Video

Author unknown

2020

Assignments (and rubrics) Molecular Neurobiology

During the course students are assessed on different skills and assignments Practical skills: Students work together as a labgroup in the lab to perform the experiments in order to answer their research questions. They are assessed on their individual practical skills but also on their teamwork in the lab. See the rubric Practical Skills_Molecular Neurobiology for the complete assessment model. Academic Attitude: Students are individually assessed on their Academic Attitude (e.g. Participation, Critical Reflection, Feedback) during the entire course. See the rubric Academic Attitude_Molecular Neurobiology for the complete assessment model. Short report: Students will write a short report (scientific article format) in English about their research, together with their lab partner. In this report they discuss the results of their research group and try to answer the research question. The report typically contains an abstract (~250 words), introduction (~500 words), materials and methods (~500 words), results including figures (~1000 words), and discussion (~500 words). References are in Harvard style and are not taken into account for the total word-count, which also holds true for the figure legends. See the rubric Report_Molecular Neurobiology for the complete assessment model. Exam: Everything can be tested on the exam. The exam is mainly focused on techniques and corresponding experimental strategies (e.g. explain how you would research the following research question, using technique X). The exam material entails all information treated during this course (lectures, lab meetings, practicals, and assignments). During the exam students can make use of Alberts (The Cell) the information in Labbuddy and ExperD, and their labjournal. The exam is in Dutch. See Practice exam and answering model_Molecular Biology for example questions and an answering model Presentation: During the course the students give several presentations in English (Journal Club, Lab meeting, and Final presentation). The presentations are assessed as a whole and as a group. However, a student could receive a different grade than the rest of the group by standing out positively or negatively during the presentations. See the rubric Presentation_Molecular Neurobiology for the complete assessment model. Journal Club: During the journal club two papers are discussed, a paper about ALS research and a paper about PD research, by means of a presentation by an ALS-group and a PD-group respectively. Everyone is expected to critically read and analyse both papers. They can make use of PerUsAll to analyse the paper as a group (see PerUsAll link below) Students need to think about whether they agree with the conclusions drawn by the authors, the chosen methods, the analysis of the results, and whether they would do anything differently. The presentation should at least contain; the research question/hypothesis of the paper, the main results (discuss the key figures of the article), the drawn conclusions, and the future research that is suggested. The presentation takes 30 minutes with 10 minutes discussion. The papers can be found under Papers Journal Club Molecular Neurobiology. Lab meetings: During the lab meetings the PD- and ALS-groups discuss their results and research strategy. Students present their research question, hypothesis, experimental design, and expectations backed-up by current literature. They also present and evaluate the results of the previous week and discuss their research strategy of the coming week. The other people present during the lab meeting can give feedback on the analyses and research strategy in order to improve the quality of the research. Lab meetings are held in English and take about one hour per group. Final Presentation: During the final presentation students present their research of the last weeks and the main conclusions in a scientific presentation. The presentation contains a short introduction of the subject, the experimental strategy and most important materials and methods, results, conclusion and a critical evaluation of the results in light of the current literature. The final presentation is 30 minutes with 10-15 minutes room for discussion.

Document

Simone Mesman

November 28, 2016

Societal Aspects of the Biobased economy

UASDocument

David Louwrier

May 1, 2021

Radboud Science Podcast | Medicijnen afleveren op specifieke plaats in het lichaam

Medicijnen geven meestal bewerkingen. Zeker bij ziektes als kanker, zijn de bijwerkingen van chemotherapie vaak groot. Hoe mooi zou het zijn als er een methode ontwikkeld wordt waarmee medicijnen alleen op de aangedane plaats in het lichaam afgeleverd zouden kunnen worden. De patiënt krijgt dan veel minder bijwerkingen én je kunt een hogere dosis van het medicijn toedienen. In het lab van Kim Bonger, doen ze fundamenteel onderzoek naar de werking van cellen en proberen ze bovengenoemde methoden te ontwikkelen. In deze aflevering vertelt Kim meer over de verschillende onderzoeken in haar lab. Ze gaat in op haar loopbaan en geeft ze ons een inkijkje in het lange proces wat volgt voor dergelijke methoden op patiënten getest kunnen worden. Daarnaast wijst ze ons op het belang van fundamenteel onderzoek en blikt ze vooruit op wat ze over 20 jaar bereikt hoopt te hebben. Over Radboud Science Podcast In de Radboud Science Podcast nemen wij je mee in het allernieuwste onderzoek van de Faculty of Science van de Radboud Universiteit in Nijmegen. Van onzichtbaar kleine deeltjes tot oneindig grote zwarte gaten, van het leven op aarde tot de ins en outs van de online wereld – geen enkel wetenschappelijk onderwerp wordt geschuwd. Elke maand vragen we onderzoekers de hemd van het lijf over hun onderzoek en brengen we je op de hoogte van de laatste inzichten. Muziek: Clemens Wijers Music Productions

Audio

Kim Bonger